Accutase composition. Composition of Accutase Accutase contains no mammalian or bacterial components and is a natural enzyme mixture with proteolytic and collagenolytic enzyme activity. Accutase ® is the gentle replacement for Trypsin in all adherent cell cultures and especially when working with sensitive cell types. Accutase® is a non-mammalian, non-bacterial ready-to-use solution. Accutase is direct replacement for trypsin cell detachment solution and does not require enzyme inactivation with serum. cancer research, and sensitive cell culture applications. This offers a gentle detachment StemPro Accutase is a gentle cell dissociation solution for routine detachment of cells from tissue culture plasticware or adhesion coated plasticware. This solution of proteolytic and collagenolytic enzymes comes ready-to-use and has been shown to work effectively across a wide variety of cell types. Composition Accutase ® enzymes (activity Accutase ® is the gentle replacement for Trypsin in all adherent cell cultures and especially when working with sensitive cell types. 15 g/l Na2HPO4) containing 0. It is free of mammalian and bacterial origin and requires no inactivation step during passaging of cells. It does not contain mammalian- or bacterial-derived This protocol describes the culture of human pluripotent stem cells (PSCs) under feeder-free conditions in a commercially available, chemically defined, growth medium, using Matrigel as a substrate and the enzyme solution Accutase for single-cell The first requirement of our assay qualification algorithm was a mononuclear cell viability post-processing of ≥80% per brain or spinal cord sample. Cell lines tested for StemPro Accutase reagent application include human embryonic stem cells, human mesenchymal stem cells, human neural stem Composition of Accutase Accutase contains no mammalian or bacterial components and is a natural enzyme mixture with proteolytic and collagenolytic enzyme activity. Accutase performs exceptionally well in detaching cells for the analysis of cell surface markers, virus growth assay, quiescence assays by Accutase® is a ready-to-use cell detachment solution containing proteolytic and collagenolytic enzymes. 2 g/l KH2PO4, 8 g/l NaCl, and 1. It contains proprietary marine-origin enzymes with Use ACCUTASE™ for routine cell detachment from plasticware. Accutase - Enzyme Cell Detachment Medium. Accutase™ is a mix of proteolytic and collagenolytic enzymes and serves as a direct replacement for trypsin and collagenase applications. Nevertheless, trypsin solutions attack the cell surface and affect cell viabil Dec 16, 2024 · Composition of Accutase and Trypsin: A Crucial Comparison for Cell Detachment When embarking on cell-based research, the choice of cell detachment technique is of paramount importance. PAN-Biotech produces and distributes Accutase ® under license of the patentee Innovative Cell Technologies. 2 g/l KCl, 0. The Accutase® solution does not contain mammalian or bacterial-derived products. Shelf life: 24 months Composition: Accutase® enzymes in Dulbecco’s Phosphate Buffered Saline (0. Accutase is Shelf life: 24 months Composition: Accutase® enzymes in Dulbecco’s Phosphate Buffered Saline (0. Accutase solution (in DPBS without Ca++ or Mg++) contains proteolytic and collagenolytic enzymes for cell detachment. StemPro Accutase reagent is a gentle cell dissociation solution used for the detaching of cells while maintaining high cell viability as compared to animal-origin enzymes such as trypsin. 5 mM EDTA•4Na and 3 mg/l Phenol Red. Available in 100 mL Product information StemProTM AccutaseTM Cell Dissociation Reagent is a ready-to-use cell detachment solution of proteolytic and collagenolytic enzymes. Accutase performs exceptionally well in detaching cells for the analysis of cell surface markers, virus growth assay, quiescence assays by Accutase is useful for the routine detachment of cells from standard tissue culture plasticware and adhesion coated plasticware, including our selection of Advanced Surface coated plasticware. Description AccutaseTM is a cell detachment solution comprised of collagenolytic and proteolytic enzymes and does not contain mammalian or bacterial derived products. Accutase ® is a non-mammalian, non-bacterial ready-to-use solution. Accutase® Cell Detachment Solution - Accutase® is a cell detachment solution containing proteolytic and collagenolytic enzymes, which are neither mammalian nor bacterial derived. Dec 16, 2024 · Composition of Accutase and Trypsin: A Crucial Comparison for Cell Detachment When embarking on cell-based research, the choice of cell detachment technique is of paramount importance. Accutase®: A gentle, mammalian-free cell dissociation solution that efficiently detaches adherent cells while preserving cell viability and surface epitopes. Ideal for stem cell. Jan 24, 2023 · For cell dissociation - a gentle alternative to trypsin The detachment of cells from culture surfaces for further cultivation or cell analysis is an unavoidable step in cell culture. Composition Accutase ® enzymes (activity Accutase is a ready to use cell detachment solution, which is non-mammalian, non-bacterial replacement for all applications of trypsin. Accutase does not contain The enzymes trypsin and accutase (Accutase ®, Innovative Cell Technologies) are frequently used for detaching adherent cells. We are the sole manufacturers of Accutase®. Exclusive and for the whole of Europe. Cell lines tested for StemPro Accutase reagent application include human embryonic stem cells, human mesenchymal stem cells, human neural stem Accutase® - the cell detachment solution that is gentle enough for use on sensitive cell types. StemProTM AccutaseTM Cell Dissociation Reagent does not contain mammalian or bacterial derived products and is useful for the routine detachment of cells from standard tissue culture plasticware and adhesion coated plasticware including Background Accutase is a cell detachment solution of proteolytic and collagenolytic enzymes that is useful for the routine detachment of cells from standard tissue culture plasticware and adhesion coated plasticware, including SmartPlasticTM. Aditionally, AccutaseTM performs well when detaching cells for the analysis of many cell surface markers using flow cytometry and Accutase® is the gentle replacement for Trypsin in all adherent cell cultures and especially when working with sensitive cell types. Accutase® Cell Detachment Solution Accutase® is an effective cell detachment solution for the routine detachment of cells from standard tissue culture plasticware as well as enhanced surface and advanced surface coated cultureware. Trypsin cleaves peptides after lysine or arginine residues that are not followed by proline and degrades most cell surface proteins depending on the incubation time. Fisher Scientific - Invitrogen Accutase Enzyme Cell Detachment Medium performs exceptionally well in detaching cells for analysis. Accutase ® solution (in Dulbecco′s phosphate-buffered saline (DPBS) without Ca++ or Mg++) contains proteolytic and collagenolytic enzymes for cell detachment. Read over the most frequently asked questions that we receive about Accutase and Accumax and find the answers you're looking for. When compared to trypsin, Acctuase™ can be used at far lower concentrations than traditional trypsin solutions. Accutase is preferred over trypsin for cell detachment as it helps to detach cells from culture vessels with StemPro Accutase reagent is a gentle cell dissociation solution used for the detaching of cells while maintaining high cell viability as compared to animal-origin enzymes such as trypsin. (A) Brains and (B) spinal cords from individual mice with experimental autoimmune encephalomyelitis scores >2 were dissociated with Percoll PLUS, Neural Tissue Dissociation Kit, or incubated with accutase or papain for 15, 30, or 60 minutes . It serves as an alternative to trypsin/EDTA for cell passages. Accutase is useful for cell detachment and for preparing single-cell suspensions from clumped cell populations for sub-culturing cells, analytical studies, and for accurate cell counting. Conventional dissociation reagents such as trypsin or trypsin/EDTA solutions are widely used as common tools in routine cell culture. Accutase® is a ready-to-use cell detachment solution containing proteolytic and collagenolytic enzymes. Accutase is useful for creating single-cell suspensions from clumped cell cultures for accurate cell counting. Accutase® , Cell Detachment Solution, is a ready-to-use cell detachment solution of proteolytic and collagenolytic enzymes. Additionally, it can be used on cells in suspension to reduce clumping for accurate counting. It does not contain mammalian- or bacterial-derived products. Useful for the routine detachment of cells from standard tissue culture plastic ware and adhesion coated plastic ware, and polymers. AccutaseTM is a replacement for trypsin solution and can be used for the passaging of cell lines. It is useful for the routine detachment of cells from standard tissue culture plasticware and adhesion- coated plasticware. It is useful for the routine detachment of cells from standard tissue culture plasticware and adhesion coated plasticware. eywu0juup1tbjigf0e5rd2d5sakzsipqsi6qnmjhj5gcxwo